Chx western blot
Web100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog number: C7698) ... Draw region of interest on top of the western blot bands and quantitate its total grey signal (this can be done in Image J and any other image analysis software). The values of total thresholded areas are measured; a ratio of Flag-Slug to its β-actin is ... WebWestern blot analysis of extracts from Jurkat cells, untreated (-) or treated with increasing concentrations of Cycloheximide (24 hr), using PARP …
Chx western blot
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WebDec 4, 2013 · The cells were collected and lysed, and the 15 μg of these lysates was subjected to Western blotting. For validation of quantitative range of Western blotting, 20, 10, 5 and 2.5 μg of TET3 CD sample without CHX were applied. Band intensities of OGT measured by ImageJ were indicated and also plotted (lower panel). WebJan 17, 2008 · Western blot result showed that both human and murine IFN-β productions of Hela cells and MCA38 cells were mildly induced after 9 h of poly I:C/CHX treatment (Fig 5A). Neutralization of IFN-β with …
WebCommunicate with your doctor Get answers to your medical questions from the comfort … WebWestern blotting is typically performed by probing the blocked membrane with a primary antibody that recognizes a specific protein or epitope on a group of proteins (e.g., SH2 domain or phosphorylated tyrosine). The choice of a primary antibody for a western blot will depend on the antigen to be detected and what antibodies are available to ...
WebApr 10, 2024 · CHX did not trigger any significant loss of Agp2-GFP in the absence of the Brp1 protein; however, the role of Brp1 in this process remains elusive. ... The membranes were incubated with the Thermofischer Western blot ECL solution as per the manual and were visualized using Pierce™ ECL Western Blotting Substrate (Thermofisher … WebJul 23, 2024 · This antibody was used at 1:500 dilution in western blot analysis. To detect the endogenous ubiquitin using a anti-Ub (P4D1, Santa Cruz Biotechnology) antibody, antigen retrieval was performed by …
WebJun 7, 2024 · L Western blot analysis of PD-L1 expression in STUB1-knockdown H1299 or SPC-A-1 cells with or without HA-ENO1 transfection. M H1299 or SPC-A-1 cells were transiently transfected with the indicated ...
WebApr 28, 2024 · The name, ‘western’ blot, was first coined by Dr. Burnette in 1981 after … lithonia ibg pdfWebFeb 10, 2024 · d Western blot analyses of DUSP6 levels in PBLD-upregulated and PBLD-downregulated cells, which were treated with 20 µg/mL CHX for the indicated periods of time. imvu download and install historyWebFeb 3, 2024 · (A) Treatment of glioma cells with CHX, western blot displayed that TGF-β could stabilize the expression of Sox9 protein. (B) Glioma cells treated with LY2109761 showed decrease of Sox9 protein, … imvu download and install freeWebMay 3, 2024 · The Western blot test is an antibody test that’s done on a blood sample. … imvu desktop for windows 10WebJun 1, 2012 · Western blotting detected cyclin B1 concentrations of 73, 53 and 66%, respectively, ... (CYC) after mouse COCs were cultured in basic medium for 0, two, or 3.5 h, following preculture for 3 h with eCG + CHX. Results of western blot analysis. The levels of cyclin B1 in freshly collected GV oocytes before preculture (FGV) were set as 100%, and ... imvu download historialWebJul 23, 2024 · Comparatively, when LINREP-modified U87 and U251 cells were treated with CHX, western blot analyses indicated that knockdown of LINREP significantly expedited the degradation rate of PTBP1 (Fig ... imvu download classicoWebJan 1, 2015 · 100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog … imvu download apk free