Cisplatin ic50 a549

WebApr 8, 2024 · Lung cancer is the leading cause of cancer‑associated death worldwide and exhibits intrinsic and acquired therapeutic resistance to cisplatin (CIS). The present study investigated the role of mTOR signaling and other signaling pathways after metformin (MET) treatment in control and cisplatin‑resistant A549 cells, mapping pathways and possible … WebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 ... USA; CRM‐CCL‐185), A549 cisplatin‐resistant (A549/DDP; National Infrastructure of Cell Line Resource, Beijing, China; 1101HUM‐PUMC000519), and human lung bronchial epithelial 16HBE cells (Shanghai Fuheng Biotechnology Co., Ltd. China; …

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WebAug 6, 2024 · The half-maximal inhibitory concentration (IC50) of A549 and A549/DDP cells was determined using CCK-8 assay. Further, circRNA profiles and differentially expressed genes in A549 and A549/DDP cells were characterized by deep sequencing and cell proliferation was measured using MTS assay. Cell cycle progression was analyzed using … WebNext, the highest cisplatin IC50 was found in CAFs, and A549 cells had the lowest IC50 value . The ... Cell viability of A549 and HCC827 cells treated with exosomes (25 μg/mL) derived from cisplatin-treated A549-R cells or CAFs. Control exosomes (Exo-control) derived from A549 or HCC827 cells’ own culture mediums. ... bishop whitehead instagram https://atucciboutique.com

Inhibitory concentrations 50 (IC50) and CI95 for cisplatin and …

WebFeb 28, 2024 · All of these new complexes have been tested on three human cancer cell lines (A549 lung, SKOV3 ovarian, and MCF-7 breast cancer cell lines) and studied for … Web灵芝总三萜与紫杉醇或与顺铂在对人源肿瘤细胞细胞毒性中的相互作用_英文_.pdf WebMar 22, 2024 · The drug’s IC 50 values indicated that the cells had acquired a stable CDDP-resistance phenotype, with the IC 50 values of the A549/DDP cells remaining … bishop whitehead robbed

Through oxaliplatin resistance induction in colorectal cancer …

Category:Full article: CAFs-Derived Exosomal miRNA-130a Confers Cisplatin ...

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Cisplatin ic50 a549

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WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were … WebMay 11, 2016 · A549 cells. After 156 hrs of monitoring, the diameter of A549 spheroid controls decreased by a total of 141 μM. During that time, spheroids treated with 20 and 40 μM of DXR also decreased, shrinking by −206 μM and −178 μM, respectively, the rate of shrinkage was faster in the treated spheroids compared with the controls.

Cisplatin ic50 a549

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WebFor A549, except for II, the other complexes exhibited moderate cytotoxicity. For non-tumorigenic ligeages, the complexes were less toxic than cisplatin for MCF-10A, with emphasis on V, which was inactive. In addition, all complexes were more selective than cisplatin at A2780-cis and for MCF-7, V exhibited selectivity index > 6. WebInhibitory concentrations 50 (IC50) and CI95 for cisplatin and compound 12 in HCT116 and A549 cells. Source publication +12 Virtual Screening and Biological Evaluation of Inhibitors Targeting...

WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug … WebNational Center for Biotechnology Information

WebDec 29, 2009 · Fig. 3B confirms that, unlike cisplatin, a conventional chemotherapeutic agent, mitaplatin selectively induced cell death in human cancer A549 cells, but not in normal MRC-5 cells under the similar treatment conditions. To address whether the selective killing of cancer cells by mitaplatin might be a consequence of its selective uptake, we ... WebIn A549 cells, the IC50 concentration of cisplatin resistant cells was determined as 23.60 µM compared to 1.58 µM in the original parent cell line, a 15-fold increase in the …

WebJul 17, 2024 · Importantly, we show that treatment of cancer cells with concentrations of cisplatin that result in 50% effect (i.e., IC50) in multiwell plate assays trigger the emergence of growth arrested cells that exhibit highly enlarged morphology, remain viable and adherent to the culture dish, and metabolize the tetrazolium salt 3-(4,5-dimethylthiazol-2 ...

WebIC 50 values for cisplatin, curcuminoid, and combination treatments in A549 cell culture. Standard deviation values are provided after the ± symbol for each inhibitory concentration value. Cell... bishop whitaker photosWebMar 17, 2024 · To establish the cisplatin-resistant cell line, A549-R, A549 cells were treated with a stepwise increasing concentration of cisplatin (Sigma-Aldrich, USA) as previously described. 29, 30 The CAFs and normal fibroblasts (NFs) were isolated from NSCLC tissues and adjacent normal tissues by primary culture, as previously described, respectively. … bishop whitehead preachingdark was the night filmWebFeb 3, 2024 · A549 and A549/CDDP cells were treated with 1/2 IC50 of cisplatin and/or PD‐0332991 for 24 h. Then cells were homogenized in RIPA buffer (50 mM Tris–HCl, pH … bishop whitehead churchWebMar 22, 2024 · The IC50 (drug concentration producing 50% growth inhibition) was calculated with GraphPad Prism Version 5.0 Software. Cell proliferation assay Cell … dark was the night cold was the ground hymnWebJan 3, 2003 · The concentration of Gleevec to inhibit 50% cell growth (IC50) was estimated to be around 2–3 μM (Figure 1), whereas the IC50 of cisplatin on A549 cells was … dark was the night creatureWebNov 19, 2013 · A549 cells were exposed to cisplatin (CDDP) at an IC50 dose (12 µM) or a range of carboplatin (CBCDA) doses and total intracellular ROS levels were measured … dark was the night actor lukas